DiR小动物活体成像荧光试剂

DiR小动物活体成像荧光试剂 下载说明书

产品名称: DiR(DiIC18(7)1,1’-dioctadecyltetramethylindotricarbocyanine Iodide)

分子式/分子量:C63H101IN2=1013.4

最大吸收波长/发射波长: 748/780 nm

推荐滤光片设置: 710 ex/760 em

结构式：

Figure 1. T-cells isolated from the spleen were fluorescently stained with DiR and i.v. injected (5x106 cells/mouse) into a Nu/Nu mouse. Images above taken 24hrs post injection with IVIS Spectrum show cells homing to the spleen.

DiR染料是亲脂性的近红外花菁荧光染料，可以用来染细胞膜和其它脂溶性生物结构。18个碳的长链插入细胞膜中从而对细胞进行染色, 而细胞间的染料转移可以忽略不计。DiR的发射的是近红外荧光可以穿透细胞和组织，在活体成像中用来示踪。

DIR一般对原代细胞进行荧光染色并可进行活体成像分布观察, (例如下列细胞embryonic stem cells, bone marrow derived stem cells, adipose derived stem cells, lymphocytes and erythrocytes)